![]() ![]() With CFL-P2200, samples were placed on a dedicated glass slide and were stirred by air flows. Other than these applications, it can principally be used for any other colored liquids because the effect of light absorption was effectively minimized.Īlthough the newer CFL-P2200 has many advantages over the older CFL-C2000 or other conventional methods, we noticed that it is still in need of more improvements for measuring blood samples easily and reliably. /OCl − and diseases, such as Alzheimer's disease 10, arteriosclerosis 11, and hypertension 12 with the rodent models.This newer system was recently applied to studying the correlative relationships between O 2 − and OCl − elicited in a small amount of blood samples by using CL and FL detecting reagents.By improving the system, we have recently developed CFL-P2200 9 and demonstrated that it can semi-automatically detect ROS and hROS, i.e., O 2 − Previously we developed a simultaneous chemiluminescence (CL) and fluorescence (FL) monitoring system CFL-C2000 8. Therefore, development of technologies for monitoring ROS and hROS produced by these cells would contribute to study oxidative stress. In recent years, involvements of ROS in various diseases have increasingly become evident 4, 5, 6.Ī central player in the innate immune responses, i.e., white blood cells (WBC), is known to produce a large amount of ROS enzymatically 4, 5, 6, 7. According to Lushchak, oxidative stress can be restated as imbalance between generation and elimination of ROS in biological systems 3. Therefore, intensity-based classification and mechanism-based sub-categorization of oxidative stress would be required 2, 3. ) and hydrogen peroxide, and highly reactive oxygen species (hROS) including hypochlorite ions (OCl −).Since the introduction of the concept, many scientists began to study oxidants, free radicals and antioxidants in biological processes, which revealed biological roles of reactive oxygen species (ROS) including superoxide radicals (O 2 − The definition was updated in 2015 as “redox signaling/control disrupting and molecular damaging imbalance between oxidants and antioxidants” 2. Helmut Sies introduced the concept of “oxidative stress” in 1985, inspired by Hans Selye’s stress theory 1. The new system could detect bodily abnormalities earlier than CRP or self-aware symptoms. By comparing these large fluctuations with the data of an inflammation marker C-reactive protein (CRP), the neutrophil count fluctuation and the timings/symptoms of abnormalities found in questionnaire, we could gain information suggesting the factors causing the large fluctuations. ![]() These parameters sometimes exceeded the healthy fluctuation range. and OCl − were nearly similar to each other and to that of the neutrophil count fluctuation.At first, healthy volunteers of both genders and of various ages showed that the fluctuation magnitudes (%) of O 2 − Using the new system, we conducted a pilot study on nominally healthy volunteers to find whether or not the monitored activities of leukocytes can distinguish more or less unhealthy conditions from healthy ones. We overcame these drawbacks by adopting a fluidic-chip container in a new system, which resulted in higher sensitivity and more stable measurements. because of open samples placed on a slide glass. ) and hypochlorite ions (OCl −) elicited from leucocytes in sample blood, but had some drawbacks, i.e., signal noise from air-flow stirring, potential biohazard risks, etc.The previous slide-glass type system could simultaneously detect reactive and highly reactive oxygen species, i.e., superoxide radicals (O 2 − ![]()
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |